The saying goes, a picture says more than a thousand words.And the human eye is the organ that provides us with about 80% of the information about the environment in which we live and how to respond to it.The main function of this organ is to capture light, form an image of the scene we see and send that image to the retina.Because the eye is made up of biological tissues, these eventually deteriorate due to natural processes that affect the quality of the image that is formed on the retina.For example, myopia, astigmatism, presbyopia.Maintaining good vision is synonymous with quality of life.Its main function is the one we carry in everyday life, from recognizing people, objects, seeing where and how to move, among others.For us it is important that the eye forms clear images on the retina through light and to be able to observe our surroundings in all its splendor.Thus, there is also the importance of forming images with high resolution in other fields, such as science.For example, in the area of ​​optical microscopy.The microscope is an optical device that amplifies the image of organisms or objects that are too small to be seen with the naked eye.The microscope is essential in the world of science since it has had an important influence on the development and formation of many of them, in the medical field the microscope has had a high impact.The study of cell biology is essential to understand the cellular relationships in the formation of tissues, and their relationship in the formation of organs.To explain the phenomena that occur in the organism of any living being, it is necessary to understand its functioning and cellular structure.Doctors use microscope lenses to detect normal and pathological cells;as well as studying the same eye to correct and treat diseases that it suffers.Over time, there has been an advance in microscopy techniques for the study of biological samples, with innovations in microscope design, sample preparation techniques, and image acquisition.The main purpose when designing an imaging optical system is that it provides us with sharp and high-contrast images, to be able to determine which image is better focused and which contains the greatest amount of information for its study.An important aspect in microscopy is the depth of field.And this is defined as the interval in distance where we can see the focused image.This interval, in turn, is related to the focused region of a thick object under analysis.Out-of-focus images begin to blur as they move away from the plane of focus.And in light microscopy, we can only get sharp images in a narrow distance range, in units of microns.The above, because the Depth of Field is small.In this way, we are limited to samples with a thickness less than or equal to .Or, several optical sections (focus planes) of the sample acquired, in order to have the image of the complete sample.Different techniques have been used over time to extend the depth of field.In general, they can be categorized into 3 main areas: 1) Through optical elements and arrangements, 2) Optical-digital hybrid systems and 3) Only digital image processing.The hybrid system makes use of optical elements called phase masks which have shown good results in the final images based on contrast and high resolution.Through the use of a digital optical system with fewer images and computational operations is required, thus extending the distance where we have focused images [3].Students of the Master's Degree in Optical Computing and the Doctorate in Optomechatronics from the Polytechnic University of Tulancingo are analyzing different profiles of phase masks and image processing algorithms.It seeks to improve the imaging technique with greater depth of field, color image processing and optimize image processing algorithms.This, in order to have color images of microscopic samples with high resolution and high contrast.Carina Toxqui Quitl and Jose Manuel Reyes Alfaro